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Aplysia neuron

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[more] [more] Scanning electron micrograph (false color) of a human induced pluripotent stem cell-derived neuron Cultured neuron with radiating dendrites The axons and dendrites of a cortical neuron Phaloidin-Atto647N stain of actin in a cultured hippocampal neuron Quadruple whole-cell patch clamp in visual cortex Hippocampal fast-spiking (FS) basket cell (BC) Expression of XFP in the Hippocampus and Cerebral Cortex Noninvasive neuroelectronic interfacing with synaptically connected snail neurons immobilized on a semiconductor chip Osmium tetroxide stained transmission electron micrograph of the many layers of membrane in myelin Hippocampal growth cones making contact Two hippocampal growth cones making a connection Hippocampal neuron growth cone pathfinding Axons forming a glomerulus in a mouse olfactory bulb Biocytin stained layer Va pyramidal neurons in rat barrel cortex Synaptic connection between an interneuron and a postsynaptic Purkinje cell in the cerebellar cortex Golgi Stained Pyramidal Cell Neuron from the abdominal ganglia of <i>Aplysia limacina</i> immunostained with the antibody anti-D-aspartic acid Green Fluorescent Protein expressing pyramidal cell in mouse cortex Sensory nerve ending of hair follicle Cerebral pyramidal neurons (Golgi stain) Astrocytes (Golgi stain) Aplysia neuron Purkinje neuron in mouse cerebellum Cultured motor neuron (red) on a monolayer of astrocytes Astrocyte Hippocampal neuron expressing monomeric Green Fluorescent Protein Neonatal rat pyramidal neuron PHF-tau-ir neurons PHF-tau-ir neurons - PV-ir axon terminals PHF-tau-ir neurons - chandelier cell axon terminals Hippocampal neuron stained for spectrin (red) and tubulin (green) Neuron transfected with doublecortin (DCX) DsRed and mannose phosphate receptor (MPR) GFP-1 Laser confocal images of neurons in hippocampal slice gene gun transfected with DCX DsRed and μ1A GFP Simultaneous patch-clamp recording from a cortical pyramidal neuron Microelectrode Dye in Purkinje Cell CA1 pyramidal neurons from FVB P12 mouse Lucifer Yellow labled pyramidal neuron and TH-ir fibers (blue) Neuron transfected with doublecortin (DCX) DsRed and mannose phosphate receptor (MPR) GFP 2 Pyramidal neurons in developing rat visual cortex
Animated BrainBow CUBIC-HV Fluorescent Myelin Populations Recording and Manipulation Single Cells  × Synapses

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Second-harmonic generation microscopy image of a primary cultured Aplysia neuron stained with the membrane dye DHPESBP. The signal is modulated by membrane potential and was found to be capable of recording action potentials with 0.6 μm and 0.833 msec spatiotemporal resolution. The high-resolution and deep tissue imaging capability of this nonlinear microscopy technique should prove valuable to future electrophysiology studies.


Technique

Second harmonic generation microscopy


Citation

Optical Recording of Action Potentials with Second-Harmonic Generation Microscopy, Daniel A. Dombeck et al., The Journal of Neuroscience, January 28, 2004, 24(4):999–1003; doi:10.1523/JNEUROSCI.4840-03.2004