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Ex vivo cortical electroporation

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Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP+ (green) pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ). Red channel: CTIP2 (red; layer 5 and 6a marker).


Citation

Neuron 48:45-62., Randal Hand et al., Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex (2005)