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Expression of XFP (= red, green, yellow or cyan fluorescent proteins) in the Hippocampus and Cerebral Cortex of transgenic mouse

(a–c) Hippocampal pyramidal cells express YFP in most lines examined, but most cells of this class are positive in some lines (such as thy1- YFP line H, [a]), whereas only a small fraction of cells are positive in others (such as thy1-GFP line M, [b]). When only a few cells are labeled, dendrite spines are clearly visible (c). (d–f) In cerebral cortex, neurons are labeled in multiple laminae of most lines (such as thy1-YFP-G, [a]). In a few lines, in contrast, expression is largely restricted to neurons with somata in specific layers—layer V in thy1-YFP line H (e) and layer VI in thy1-GFP line I (f).


Technique

Selective expression of red, green, yellow or cyan fluorescent proteins in neurons by oocyte DNA injection


Location

Department of Anatomy and Neurobiology and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, USA


Citation

Guoping Feng, Rebecca H. Mellor, Michael Bernstein, Cynthia Keller-Peck, Quyen T. Nguyen, Mia Wallace, Jeanne M. Nerbonne, Jeff W. Lichtman, and Joshua R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neurotechnique 28 41–51 (2000)”